Implementation Blueprint
Goal alignment: Sleep, Longevity, Stress Resilience.[512,660,884, etc.]
Dose guide: 3 g 30-60 minutes before bed.[512,660,884, etc.]
Category
Amino Acid
Evidence Tier
HIGH
Daily Guide
3 g 30-60 minutes before bed
Supplement
Supports inhibitory neurotransmission and collagen synthesis.[2715,2809,3276, etc.]
Superscript reference numbers link to the Sources bibliography.
Composite Rating
Blends evidence depth, community direction, safety friction, and completeness.
Evidence Intensity
100/100
High depth from human/RCT/meta counts
Community Direction
18
Positive Lean • confidence Minimal
Safety Friction
100/100
High signal from adverse/interaction markers
Data Completeness
100%
Studies 15 • mentions 4 • links 25
Not medical advice. This product is for educational and informational purposes only. It does not diagnose, treat, cure, or prevent any disease and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the advice of a qualified healthcare provider with any questions regarding a medical condition.
FDA disclaimer: These statements have not been evaluated by the Food and Drug Administration. Dietary supplements are not intended to diagnose, treat, cure, or prevent any disease. Results may vary. Not intended for use by pregnant or nursing women, individuals under 18, or those with known medical conditions without physician consultation.
If you are experiencing a medical emergency, call 911 or your local emergency number immediately.
Goal alignment: Sleep, Longevity, Stress Resilience.[512,660,884, etc.]
Dose guide: 3 g 30-60 minutes before bed.[512,660,884, etc.]
Category
Amino Acid
Evidence Tier
HIGH
Daily Guide
3 g 30-60 minutes before bed
Clinical Depth
Humans: 2,444 • RCTs: 462 • Meta: 58
Cohort / Real-World
Cohort signals: 0
Safety Markers
Adverse signals: 4,418 • Interactions: 12,189
Freshness, trend, and study timeline for this item
Status
Trend
→ Stable evidence base
Depth
7726 total studies · 3 recent
Timeline
Animal-only studies
38,372
Interaction signals
12,189
In vitro / tissue
10,513
Total indexed
7,726
Safety adverse signals
4,418
Human studies
2,444
Weighted direction score: 18 (Positive Lean)
High when >=2 human meta-analyses, or >=5 human RCTs with substantial human literature volume.
Animal-only studies
38,372
Interaction signals
12,189
In vitro / tissue
10,513
Total indexed
7,726
Safety adverse signals
4,418
Human studies
2,444
Randomized trials
462
Meta-analyses
58
Distribution of evidence by study design strength
Evidence Strength: High
Composite of study volume, RCTs, and meta-analyses
PMID 36728680 • 2023 Jul 10
The effects of oral ferrous bisglycinate supplementation on hemoglobin and ferritin concentrations in adults and children: a systematic review and meta-analysis of randomized controlled trials.
Nutr Rev
Open PubMedPMID 39433088 • 2025 Jan
Effect of Soy Isoflavones on Measures of Estrogenicity: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.
Adv Nutr
Open PubMedPMID 28642459 • 2017 Jun 22
Meta-analysis of Soy Consumption and Gastrointestinal Cancer Risk.
Benefit reports: 2
Adverse reports: 1
No-effect reports: 0
Mixed reports: 1
Unclear reports: 0
Signal confidence: 0.00
Benefits
Adverse
No Effect
Often combined with magnesium/theanine in sleep stacks. | Step2: Insufficient empirical signal for a directional adjustment.
Reddit evidence: 0 mentions (confidence 0.00).
aggregate • benefit
Aggregate community signal: 4 directional mentions (benefit 2, adverse 1, no-effect 0, mixed 1).
Open mentionamazon
Amazon search for Glycine
Rating: n/a | Reviews: n/a
Open productEvidence Sources
RCT • 2019 • Adults
Small healthy adult cohorts.
Outcome: Improved sleep quality and next-day fatigue.[2715,2809,3276, etc.]
PUBMED • Humans + preclinical triage
Counts aggregated from automated literature sweep and tiering rules.
Outcome: Tier HIGH; humans 2444, RCTs 462, meta-analyses 58.[2715,2809,3276, etc.]
Open sourceSci Rep
PMID 31915830 • 2020 Mar 1
Legume and soy intake and risk of type 2 diabetes: a systematic review and meta-analysis of prospective cohort studies.
Am J Clin Nutr
Open PubMedPMID 38828426 • 2024
Efficacy of daprodustat for patients on dialysis with anemia: systematic review and network meta-analysis.
Pan Afr Med J
Open PubMedPMID 35745197 • 2022 Jun 14
Role of Phytoestrogen-Rich Bioactive Substances (Linum usitatissimum L., Glycine max L., Trifolium pratense L.) in Cardiovascular Disease Prevention in Postmenopausal Women: A Systematic Review and Meta-Analysis.
Nutrients
Open PubMedPMID 35975308 • 2023 Jan 26
Supplementing Glycine and N-Acetylcysteine (GlyNAC) in Older Adults Improves Glutathione Deficiency, Oxidative Stress, Mitochondrial Dysfunction, Inflammation, Physical Function, and Aging Hallmarks: A Randomized Clinical Trial.
J Gerontol A Biol Sci Med Sci
Open PubMedPMID 36253903 • 2023 Jan 1
A dietary intervention for vasomotor symptoms of menopause: a randomized, controlled trial.
Menopause
Open PubMedPMID 27852613 • 2017 Jan
Vitamin C-enriched gelatin supplementation before intermittent activity augments collagen synthesis.
Am J Clin Nutr
Open PubMedPMID 38717445 • 2024 Jan-Dec
Metabolome-associated psychological comorbidities improvement in irritable bowel syndrome patients receiving a probiotic.
Gut Microbes
Open PubMedPMID 36094342 • 2023 Feb 1
Nutritional Modulation of Sleep Latency, Duration, and Efficiency: A Randomized, Repeated-Measures, Double-Blind Deception Study.
Med Sci Sports Exerc
Open PubMedPMID 30971437 • 2019 Aug
Dietary supplementation with inulin-propionate ester or inulin improves insulin sensitivity in adults with overweight and obesity with distinct effects on the gut microbiota, plasma metabolome and systemic inflammatory responses: a randomised cross-over trial.
Gut
Open PubMedPMID 33811821 • 2021 Jun 1
Creatine promotes cancer metastasis through activation of Smad2/3.
Cell Metab
Open PubMedPMID 33783984 • 2021 Mar
Glycine and N-acetylcysteine (GlyNAC) supplementation in older adults improves glutathione deficiency, oxidative stress, mitochondrial dysfunction, inflammation, insulin resistance, endothelial dysfunction, genotoxicity, muscle strength, and cognition: Results of a pilot clinical trial.
Clin Transl Med
Open PubMedPMID 32868164 • 2020 Oct
Folic acid versus 5- methyl tetrahydrofolate supplementation in pregnancy.
Eur J Obstet Gynecol Reprod Biol
Open PubMedAmazon signal: 0 products scanned (confidence 0.00).
GNC signal: 0 products scanned (confidence 0.00) [BLOCKED].
PerimeterX / captcha block encountered.
Search fetch failed: Failed to fetch after retries: https://old.reddit.com/search.json?raw_json=1&sort=relevance&t=year&limit=12&q=%22glycine%22%20(supplement%20OR%20dosage%20OR%20stack%20OR%20side%20effects%20OR%20review)%20(subreddit%3ASupplements%20OR%20subreddit%3ANootropics%20OR%20subreddit%3ABiohackers%20OR%20subreddit%3Alongevity%20OR%20subreddit%3ANutrition%20OR%20subreddit%3AHubermanLab%20OR%20subreddit%3ASleep%20OR%20subreddit%3AFitness%20OR%20subreddit%3Aketoscience%20OR%20subreddit%3AStackAdvice)
Fallback search failed: Failed to fetch after retries: https://old.reddit.com/search.json?raw_json=1&sort=comments&t=all&limit=16&q=%22glycine%22%20(supplement%20OR%20capsule%20OR%20powder%20OR%20experience%20OR%20results%20OR%20side%20effects)
Broad search failed: Failed to fetch after retries: https://old.reddit.com/search.json?raw_json=1&sort=new&t=all&limit=16&q=glycine
Use adverse/interactions PubMed signal counts for screening; perform clinician review before high-dose or multi-drug stack use.
Imported legacy community score: 9.0/10 (heuristic mapping confidence 1.00).
Imported legacy monthly cost signal: ~$8.00.
Legacy community note: Rapidly growing popularity due to GlyNAC aging study. Users love the sweet taste and report better sleep, improved skin, and a sense of calm. One of the most cost-effective supplements available.
Community Sources
PUBMED • 2023 • Human evidence curation set
PMID 36728680 from Nutr Rev.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2025 • Human evidence curation set
PMID 39433088 from Adv Nutr.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2017 • Human evidence curation set
PMID 28642459 from Sci Rep.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2020 • Human evidence curation set
PMID 31915830 from Am J Clin Nutr.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2024 • Human evidence curation set
PMID 38828426 from Pan Afr Med J.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2022 • Human evidence curation set
PMID 35745197 from Nutrients.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2023 • Human evidence curation set
PMID 35975308 from J Gerontol A Biol Sci Med Sci.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2023 • Human evidence curation set
PMID 36253903 from Menopause.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2017 • Human evidence curation set
PMID 27852613 from Am J Clin Nutr.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2024 • Human evidence curation set
PMID 38717445 from Gut Microbes.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2023 • Human evidence curation set
PMID 36094342 from Med Sci Sports Exerc.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2019 • Human evidence curation set
PMID 30971437 from Gut.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2021 • Human evidence curation set
PMID 33811821 from Cell Metab.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2021 • Human evidence curation set
PMID 33783984 from Clin Transl Med.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open sourcePUBMED • 2020 • Human evidence curation set
PMID 32868164 from Eur J Obstet Gynecol Reprod Biol.
Outcome: See linked PubMed abstract for methods and outcomes.[2715,2809,3276, etc.]
Open source